Journal: Frontiers in Pharmacology

Article Title: Postbiotic muramyl dipeptide alleviates colitis via activating autophagy in intestinal epithelial cells

doi: 10.3389/fphar.2022.1052644

Figure Lengend Snippet: Antibody information.

Article Snippet: ZO-1 , Bioss inc. , bs-1329R , WB(1:1000).

Techniques:

Journal: bioRxiv

Article Title: Effects of Alternative Splicing-Specific Knockdown of Tjp1 α+ by Rbm47 on Tight Junctions Assembly during Blastocyst Development

doi: 10.1101/2023.07.18.549609

Figure Lengend Snippet: (A) The position of both isoforms of Tjp1 (exon11/12; α+/α-), the α-domain (exon20/21) and two Tjp1 α-specific (exon 19/21; w/o α and alternatively spliced exon 27/28; α-) primers used for cDNA amplification of Tjp1 isoforms. Arrows mark the position and direction of primers used for cDNA synthesis. Transcription levels of Tjp1 α+/-, Tjp1 α variant (B), and Tjp1 α+/-, Tjp1 α-, Rbm47 (C). Transcription levels of Tjp1 α+/α-, Tjp1 α-, and Rbm47, shown as relative quantification (RQ). Error bars represent mean ± standard error.

Article Snippet: Primary antibodies against RBM47 (HPA006347, Sigma-Aldrich), TJP1 α+ (HP9044; Hycult Biotechnology, Uden, Netherlands) and TJP1 α-(HP9045; Hycult Biotechnology) were diluted and incubated with the embryos overnight at 4°C.

Techniques: Amplification, Variant Assay

Journal: bioRxiv

Article Title: Effects of Alternative Splicing-Specific Knockdown of Tjp1 α+ by Rbm47 on Tight Junctions Assembly during Blastocyst Development

doi: 10.1101/2023.07.18.549609

Figure Lengend Snippet: (A) Expression and localisation of Tjp1 α+ and Tjp1 α-in the Rbm47 KD blastocyst. (B) Gene expression patterns of Tjp1 variants from 8-cell to hatching blastocyst (C) Proximity ligation assay (PLA) showing the interaction between Tjp1(mainly Tjp1 α-) and Ocln in blastocysts. 8C(8-cell), 8c-cmp (compacted 8-cell), Mo (Morula), Lt-Mo (Late morula), E-Bl (early blastocyst), Ex-Bl (Expanding-Bl), H-Bl (Hatching blastocyst). Antibodies host: Rabbit (Rb), Guinea pig (GP), mouse (m).

Article Snippet: Primary antibodies against RBM47 (HPA006347, Sigma-Aldrich), TJP1 α+ (HP9044; Hycult Biotechnology, Uden, Netherlands) and TJP1 α-(HP9045; Hycult Biotechnology) were diluted and incubated with the embryos overnight at 4°C.

Techniques: Expressing, Proximity Ligation Assay

Journal: Viruses

Article Title: Effects of US7 and UL56 on Cell-to-Cell Spread of Human Herpes Simplex Virus 1

doi: 10.3390/v15112256

Figure Lengend Snippet: Phenotypic difference between HB94 and HN19. ( A ) Difference in syncytial size. Vero cells (3 × 10 5 cells/well) were infected by 50 PFU HB94 or HN19 strains and cultured for 3 days. The white squares shows the enlarged plaque image (right) with a bar of 1 mm. ( B ) Difference in syncytia size. Vero cells were infected by HSV-1 at 0.01 MOI cultured for 24 h. The ZO−1 protein indicates the ability of syncytia. ( C ) One-step growth curve at 5 or 0.01 MOI. The cellular supernatants were harvested at 12, 24, 36, 48, and 72 h.p.i., and the viral titers were detected by plaque assay. ( D ) Growth curve of the ratio of virions entering cells. Vero cells (3 × 10 5 cells/well) were infected by 120 PFU HB94 or HN19 strains at 4 °C for 1 h. Virus supernatant was replaced by DMEM medium and cultured at 37 °C for viral entry. Then, the medium was replaced by low-PH citrate buffer solution at 0, 10, 20, 30, 45, and 60 min. Virions that did not enter the cell were inactivated by citric acid buffer solution. After that, the number of plaques in the experimental group and the control group was calculated to determine the proportion of virus entry. ( E ) Growth curve of gD gene copies at 5 MOI. The cellular supernatants were harvested at 2, 6, 12, 18, and 24 h.p.i., and the viral gD gene copies were detected by qPCR. Ns: not significant.

Article Snippet: An anti-ZO-1 polyclonal antibody (Beyotime, Shanghai, China) was used to detect syncytia.

Techniques: Infection, Cell Culture, Plaque Assay, Virus